1
Baron Helmut Zemo
2
Arnim Zola
3
Black Mamba
4
Red Skull
5
M.O.D.O.K.
6
Hive
7
Batroc the Leaper
8
Crossbones
9
Captain Hydra
10
Nightshade
11
Jack O' Lantern (Steven Levins)
12
Flag-Smasher
13
Scourge of the Underworld
14
Porcupine
15
William Burnside
16
Baron Heinrich Zemo
17
Viper
18
Superia
19
Hydra
20
Sin
21
Super-Adaptoid
22
Constrictor
23
Armadillo
24
Machinesmith
25
Coachwhip
26
Vermin
27
Blue Streak
28
Bushmaster
29
Anaconda
30
Baron Strucker
31
Zaran the Weapons Master
32
Asp
33
A.I.M.
34
Princess Python
35
Baron Blood
36
Power Broker
37
Sidewinder
38
Death Adder
39
Dr Faustus
40
King Cobra
41
Cutthroat
42
Slug
43
U.L.T.I.M.A.T.U.M.
44
Puff Adder
45
Moonstone (Lloyd Bloch)
46
Eel
47
Cottonmouth
48
Serpent Society
49
Golden Claw
50
Rattler
51
Commision on Superhuman Activities
52
Nuke
53
Secret Empire
54
Hate-Monger