1
Arnim Zola
2
Red Skull
3
Baron Helmut Zemo
4
Batroc the Leaper
5
Black Mamba
6
Hydra
7
Viper
8
A.I.M.
9
Crossbones
10
Hive
11
Baron Blood
12
Slug
13
Death Adder
14
Serpent Society
15
Captain Hydra
16
M.O.D.O.K.
17
Jack O' Lantern (Steven Levins)
18
Scourge of the Underworld
19
Power Broker
20
Cutthroat
21
Coachwhip
22
Nightshade
23
Machinesmith
24
Dr Faustus
25
Sin
26
Vermin
27
Eel
28
Bushmaster
29
Constrictor
30
Rattler
31
Flag-Smasher
32
Blue Streak
33
Nuke
34
Armadillo
35
U.L.T.I.M.A.T.U.M.
36
Baron Strucker
37
Hate-Monger
38
Asp
39
Princess Python
40
Puff Adder
41
Anaconda
42
Super-Adaptoid
43
William Burnside
44
Cottonmouth
45
Zaran the Weapons Master
46
Superia
47
Baron Heinrich Zemo
48
Golden Claw
49
Porcupine
50
King Cobra
51
Sidewinder
52
Moonstone (Lloyd Bloch)
53
Commision on Superhuman Activities
54
Secret Empire